*Faster 1 hour protocol
*Blue dye added to reagent to facilitate organic/aqueous phase separation
*Applications are the same as those of RNAzol
*2 ml isolates RNA from 100 mg tissue or 1 x 107 cells or 10 x 107cells
RNA-Bee (100 ml)
RNA-Bee is a complete and ready-to-use reagent for isolation of total RNA from samples of human, animal, plant, bacterial and viral origin. RNA-Bee is the improved version of the single-step method of RNA isolation (1). The improved RNA-Bee provides a fast and highly reliable method for isolating pure and undegraded RNA from a large variety of biological samples.
RNA-Bee and the single-step method are subjects of the US patent 4,843,155.
RNA-Bee is a monophase solution containing phenol and guanidine thiocyanate. A biological sample is homogenized or lysed in RNA-Bee and the homogenate/lysate is separated into aqueous and organic phase by the addition of chloroform. The subsequent centrifugation efficiently removes DNA and proteins from the aqueous phase containing RNA. The undegraded, pure RNA is obtained from the aqueous phase by the isopropanol precipitation, washing with ethanol and solubilization in an appropriate solution, The entire isolation procedure can be completed in 1 hour. The isolated RNA is appropriate for Northern blotting, poly A + selection, RT-PCR, and other molecular biology techniques.
STABILITY: RNA-Bee is stable at 2 - 8 C for at least one year from date of purchase.
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